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APPENDICES <br />City of Redwood City, Public Works Division Initial Study & Mitigated Negative Declaration <br /> <br /> 16 Blankinship & Associates, Inc. <br /> <br />loss in adults. Juveniles were too small to evaluate scale loss. Physiological condition of fish fed <br />invertebrates containing metals was compromised. <br /> <br />Woodward et al. 1995 <br />Rainbow trout (Oncorhynchus mykiss) and brown trout (Salmo trutta) were held in standard <br />laboratory water or contained metal concentrations based on 50% the U.S. EPA’s water-quality <br />criteria with concentrations of 1.1 μg/L Cd, 12 μg/L Cu, 3.2 μg/L Pb, and 50 μg/L Zn from hatching to <br />88 days of age. Three diets were provided that comprised of benthic invertebrates collected from <br />three locations on the Clark Fork River, Montana. Fish received pelleted invertebrates containing 6.5 <br />As, no Cd, 87 Cu, 6.9 Pb, and 616 Zn (all mg/g dry weight); 19 As, no Cd, 178 Cu, 15 Pb, and 650 Zn <br />(all mg/g dry weight); or 19 As, 0.26 Cd, 174 Cu, 15 Pb, and 648 Zn (all mg/g dry weight). Survival <br />was not affected for either species by any combination of water or diet. Growth of brown trout was <br />reduced in the groups receiving the diets with higher metals concentration and by exposure to metal- <br />containing water from day 26 onward in the test. In rainbow trout, no effects were seen on growth at <br />day 18, but by day 53, growth was reduced in fish exposed to higher metal concentrations in diet or <br />water. However, the rainbow trout exposed to diets with higher metals concentrations had similar <br />growth patterns regardless of whether they were also exposed to metals-containing water. Also, the <br />growth of the rainbow trout exposed to treated water and the diet with low metal concentrations <br />recovered by day 88 and were no longer significantly different from fish in untreated water. <br /> <br />Draves and Fox 1998 <br />In a reach of the Montreal River in northern Ontario contaminated from gold mine tailings, water <br />concentrations were significantly higher for Cu, Cd, and Pb, but not for Zn. Juvenile yellow perch <br />(Perca flavescens), a benthic feeding species, had significantly less food in their stomachs in the <br />contaminated reach than perch in an uncontaminated reach. However, body weights of juvenile perch <br />did not differ between the contaminated and uncontaminated reaches. Within the contaminated reach, <br />Cu body burdens were significantly negatively correlated with body weight. Concentrations of Cu in <br />Chironomidae, Hemiptera, Cladocera, Odonata, and Amphipoda were compared between reaches. <br />Concentrations in Chironomidae, Hemiptera, Cladocera, and Amphipoda were greater in the <br />contaminated reach, but Cu concentrations were greater in Odonata in the uncontaminated reach. <br /> <br />Sublethal Effects <br />Folmar 1976 <br />Rainbow trout (Oncorhynchus mykiss) fry showed strong avoidance to copper (CuSO4·5H2O) at <br />concentrations of 0.0001 to 0.01 ppm in the laboratory. <br /> <br />Folmar 1978 <br />Mayfly nymphs (Ephemerella walkeri) showed strong avoidance to copper (CuSO4·5H2O) at a <br />concentration of 0.1 ppm but not 0.001 or 0.01 ppm in the laboratory. <br /> <br />6.3.A. - Page 85